Coding

Part:BBa_K4258011

Designed by: Jake Schumacher   Group: iGEM22_NDSU   (2022-09-26)


His-meffRed-Linker-CBDfimi

Bsa1 sites for Golden Gate cloning as a CD module and Bpi1 sites for cloning into pOGG006 to immortalize the part as a plasmid. 6x Histidine tag + MeffRed from BBa_K1033920 + 10x GS Linker + Cellulose Binding Domain from Cellulomonas fimi (BBa_K863101.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 565
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 309
    Illegal NgoMIV site found at 774
    Illegal NgoMIV site found at 786
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 16
    Illegal BsaI.rc site found at 1109


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